https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19659 Wed 27 Jul 2022 13:52:25 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31116 Wed 24 Nov 2021 15:52:49 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36471 Wed 17 Nov 2021 16:29:19 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47186 Wed 14 Dec 2022 16:02:49 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26646 Wed 11 Apr 2018 17:03:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14032 Wed 11 Apr 2018 16:55:30 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22505 Wed 11 Apr 2018 16:25:30 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28269 Wed 11 Apr 2018 15:33:28 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21399 Wed 11 Apr 2018 15:17:09 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29574 Wed 11 Apr 2018 14:11:09 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:9258 Wed 11 Apr 2018 12:50:21 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:8722 Wed 11 Apr 2018 11:13:25 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15172 Wed 11 Apr 2018 10:45:36 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30115 Wed 04 Oct 2017 12:46:07 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45692 EgENOD₉₃ expression is essential for somatic embryogenesis. Abstract: Micropropagation of oil palm through tissue culture is vital for the generation of superior and uniform elite planting materials. Studies were carried out to identify genes to distinguish between leaf explants with the potential to develop into embryogenic or non-embryogenic callus. Oil palm cDNA microarrays were co-hybridized with cDNA probes of reference tissue, separately with embryo forming (media T527) and non-embryo (media T694) forming leaf explants sampled at Day 7, Day 14 and Day 21. Analysis of the normalized datasets has identified 77, 115 and 127 significantly differentially expressed genes at Day 7, Day 14, and Day 21, respectively. An early nodulin 93 protein gene (ENOD₉₃), was highly expressed at Day 7, Day 14, and Day 21 and in callus (media T527), as assessed by RT-qPCR. Validation of EgENOD₉₃ across tissue culture lines of different genetic background and media composition showed the potential of this gene as an embryogenic marker. In situ RNA hybridization and functional characterization in Medicago truncatula provided additional evidence that ENOD₉₃ is essential for somatic embryogenesis. This study supports the suitability of EgENOD₉₃ as a marker to predict the potential of leaf explants to produce embryogenic callus. Crosstalk among stresses, auxin, and Nod-factor like signalling molecules likely induces the expression of EgENOD₉₃ for embryogenic callus formation.]]> Thu 03 Nov 2022 10:03:56 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15362 Sat 24 Mar 2018 08:25:44 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14498 Sat 24 Mar 2018 08:21:40 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:10807 Sat 24 Mar 2018 08:11:50 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20947 50, p=4.02E-06), which may have clinical/biological implications.]]> Sat 24 Mar 2018 08:06:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19294 Sat 24 Mar 2018 07:56:07 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21833 HFE gene. There is evidence from both human and animal studies that HFE gene variants may affect brain function and modify risks of brain disease. To investigate how disruption of HFE influences brain transcript levels, we used microarray and real-time reverse transcription polymerase chain reaction to assess the brain transcriptome in Hfe^−/− mice relative to wildtype AKR controls (age 10 weeks, n ≥ 4/group). The Hfe^−/− mouse brain showed numerous significant changes in transcript levels (p < 0.05) although few of these related to proteins directly involved in iron homeostasis. There were robust changes of at least 2-fold in levels of transcripts for prominent genes relating to transcriptional regulation (FBJ osteosarcoma oncogene Fos, early growth response genes), neurotransmission (glutamate NMDA receptor Grin1, GABA receptor Gabbr1) and synaptic plasticity and memory (calcium/calmodulin-dependent protein kinase IIα Camk2a). As previously reported for dietary iron-supplemented mice, there were altered levels of transcripts for genes linked to neuronal ceroid lipofuscinosis, a disease characterized by excessive lipofuscin deposition. Labile iron is known to enhance lipofuscin generation which may accelerate brain aging. The findings provide evidence that iron loading disorders can considerably perturb levels of transcripts for genes essential for normal brain function and may help explain some of the neurologic signs and symptoms reported in hemochromatosis patients.]]> Sat 24 Mar 2018 07:52:17 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26945 Sat 24 Mar 2018 07:27:04 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28192 Sat 24 Mar 2018 07:23:54 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:25111 Sat 24 Mar 2018 07:17:15 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23144 mut), a model of a rare type of hereditary hemochromatosis, relative to wildtype control mice. The results were compared with our previous findings in dietary iron-supplemented wildtype mice and Hfe^−/− mice, a model of a common type of hereditary hemochromatosis. For transcripts showing significant changes relative to controls across all three models, there was perfect (100%) directional concordance (i.e. transcripts were increased in all models or decreased in all models). Comparison of the two models of hereditary hemochromatosis, which showed more pronounced changes than the dietary iron-supplemented mice, revealed numerous common molecular effects. Pathway analyses highlighted changes for genes relating to long-term depression (6.8-fold enrichment, p = 5.4 × 10⁻⁷) and, to a lesser extent, long-term potentiation (3.7-fold enrichment, p = 0.01), with generalized reductions in transcription of key genes from these pathways, which are involved in modulating synaptic strength and efficacy and are essential for memory and learning. The agreement across the models suggests the findings are robust and strengthens previous evidence that iron loading disorders affect the brain. Perturbations of brain phenomena such as long-term depression and long-term potentiation might partly explain neurologic symptoms reported for some hemochromatosis patients.]]> Sat 24 Mar 2018 07:10:33 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30639 Mon 23 Sep 2019 11:53:06 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32299 Mon 23 Sep 2019 10:12:04 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42306 n = 3/group). Those predicted to target RAS mRNAs, or that were decreased in early gestation, were confirmed by qPCR (n = 9/group). RAS protein levels were assessed by ELISAs or immuno-blotting. Microarray analysis identified four miRNAs predicted to target RAS mRNAs that were differentially expressed between 1 and 5% oxygen. Using qPCR, 15 miRNAs that target the RAS were measured in HTR-8/SVneo cells. Five miRNAs were downregulated in 1% compared with 5% oxygen. Expression of a number of RAS mRNAs (ATP6AP2, AGT, ACE and AGTR1) were increased in either, or both, 1 and 5% oxygen compared with 20% oxygen. AGT protein levels were increased in 1% oxygen compared with 5%. Further validation is needed to confirm that these miRNAs target RAS mRNAs directly and that placental development is partly regulated by oxygen-sensitive miRNAs that target RAS mRNAs. Since placental oxygen tension changes across gestation, changes in expression of these miRNAs may contribute to the transgestational changes in placental RAS expression and the resulting effects on placental development.]]> Mon 22 Aug 2022 09:00:22 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:46592 n = 33). SNP-microarray analysis identified additional aberrations in 97% of patients (32/33) compared to conventional techniques. This changed the genomic risk category of 24% (8/33) of patients. Additionally, 27% (9/33) of patients exhibited a ‘hyperdiploid’ genome, which is generally associated with a good genomic risk and favourable outcomes. An enrichment of IKZF1 deletions was observed with one third of the cohort affected. Our findings suggest the current classification system could be improved and highlights the need to use more sensitive techniques such as SNP-microarray for cytogenomic risk stratification in B-ALL.]]> Fri 25 Nov 2022 15:04:02 AEDT ]]>